Purpose(Empty Backbone) expresses sgRNA from the U6 promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51133||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||51133-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLKO.inducible
- 3′ sequencing primer O.PGK1b-R (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 51133-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-U6-sgRNA-PGK-puromycin was a gift from Xingxu Huang (Addgene plasmid # 51133 ; http://n2t.net/addgene:51133 ; RRID:Addgene_51133)
For your References section:Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. 10.1038/nmeth.2857 PubMed 24584192