This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pSIR-GFP
(Plasmid #51134)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51134 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSIR
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 8089
  • Modifications to backbone
    Replacement of the neomycin resistance gene with GFP
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Low Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    We have recognized that rare recombination evens may occur and give rise to clones with deletions (ca. 5 kb in size). If you encounter this problem, try picking more colonies (at least 10). Use 1 ml for miniprep and double-digest with Xho I and Nco I. Correct clones have 7.0 kbp + 890 bp (wrong ones are around 5 kbp). Please use the remaining 4 ml culture of the correct clones for expansion into a large scale. You may find dark colonies and pale colonies on the plate, and the pale ones are correct clones in our hand. In cloning of gBlock, the plasmid will be digested with Xho I and Hind III, for example, and the 8 kbp fragment will be purified. Therefore, the deleted clones will be removed during the purification step even if they are contaminated in the sample.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIR-GFP was a gift from Hodaka Fujii (Addgene plasmid # 51134 ; http://n2t.net/addgene:51134 ; RRID:Addgene_51134)
  • For your References section:

    Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita T, Fujii H. PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. 10.1371/journal.pone.0103084 PubMed 25051498