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3xFLAG-dCas9/pMXs-IG Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid 3xFLAG-dCas9/pMXs-IG with the following enzymes: AgeI-HF and NotI-HF.

Expected Bands
AgeI-HF: 10,303 bp
NotI-HF: 10,303 bp
AgeI-HF/NotI-HF: 5682 bp & 4621 bp

We observe the expected bands for this digest.