Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pCAG-NLS-HA-Bxb1 Citations (16)

Originally described in: Binary recombinase systems for high-resolution conditional mutagenesis.
Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P Nucleic Acids Res. 2014 Jan 9. PubMed Journal

Articles Citing pCAG-NLS-HA-Bxb1

Articles
A modular open platform for systematic functional studies under physiological conditions. Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H, Bultmann S. Nucleic Acids Res. 2015 May 24. pii: gkv550. PubMed

Associated Plasmids
A platform for functional assessment of large variant libraries in mammalian cells. Matreyek KA, Stephany JJ, Fowler DM. Nucleic Acids Res. 2017 Mar 15. doi: 10.1093/nar/gkx183. PubMed
An improved platform for functional assessment of large protein libraries in mammalian cells. Matreyek KA, Stephany JJ, Chiasson MA, Hasle N, Fowler DM. Nucleic Acids Res. 2020 Jan 10;48(1):e1. doi: 10.1093/nar/gkz910. PubMed
High-Throughput Reclassification of SCN5A Variants. Glazer AM, Wada Y, Li B, Muhammad A, Kalash OR, O'Neill MJ, Shields T, Hall L, Short L, Blair MA, Kroncke BM, Capra JA, Roden DM. Am J Hum Genet. 2020 Jul 2;107(1):111-123. doi: 10.1016/j.ajhg.2020.05.015. Epub 2020 Jun 12. PubMed
SLCO1B1: Application and Limitations of Deep Mutational Scanning for Genomic Missense Variant Function. Zhang L, Sarangi V, Ho MF, Moon I, Kalari KR, Wang L, Weinshilboum RM. Drug Metab Dispos. 2021 May;49(5):395-404. doi: 10.1124/dmd.120.000264. Epub 2021 Mar 3. PubMed
Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation. Makhija S, Brown D, Rudlaff RM, Doh JK, Bourke S, Wang Y, Zhou S, Cheloor-Kovilakam R, Huang B. ACS Chem Biol. 2021 Apr 16;16(4):671-681. doi: 10.1021/acschembio.0c00925. Epub 2021 Mar 18. PubMed
A Sort-Seq Approach to the Development of Single Fluorescent Protein Biosensors. Koberstein JN, Stewart ML, Mighell TL, Smith CB, Cohen MS. ACS Chem Biol. 2021 Sep 17;16(9):1709-1720. doi: 10.1021/acschembio.1c00423. Epub 2021 Aug 25. PubMed
Integrating thousands of PTEN variant activity and abundance measurements reveals variant subgroups and new dominant negatives in cancers. Matreyek KA, Stephany JJ, Ahler E, Fowler DM. Genome Med. 2021 Oct 14;13(1):165. doi: 10.1186/s13073-021-00984-x. PubMed
Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase. Reisman BJ, Guo H, Ramsey HE, Wright MT, Reinfeld BI, Ferrell PB, Sulikowski GA, Rathmell WK, Savona MR, Plate L, Rubinstein JL, Bachmann BO. Nat Chem Biol. 2022 Apr;18(4):360-367. doi: 10.1038/s41589-021-00900-9. Epub 2021 Dec 2. PubMed
Generation of a host cell line containing a MAR-rich landing pad for site-specific integration and expression of transgenes. Oliviero C, Hinz SC, Bogen JP, Kornmann H, Hock B, Kolmar H, Hagens G. Biotechnol Prog. 2022 Jul;38(4):e3254. doi: 10.1002/btpr.3254. Epub 2022 Apr 25. PubMed
STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Blanch-Asensio A, Grandela C, Brandao KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, Mummery CL, Davis RP. Cell Rep Methods. 2022 Sep 22;2(10):100300. doi: 10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24. PubMed

Associated Plasmids
Ultra-high throughput mapping of genetic design space. O'Connell RW, Rai K, Piepergerdes TC, Wang Y, Samra KD, Wilson JA, Lin S, Zhang TH, Ramos E, Sun A, Kille B, Curry KD, Rocks JW, Treangen TJ, Mehta P, Bashor CJ. bioRxiv. 2023 Jul 6:2023.03.16.532704. doi: 10.1101/2023.03.16.532704. Preprint. PubMed
High-throughput functional mapping of variants in an arrhythmia gene, KCNE1 , reveals novel biology. Muhammad A, Calandranis ME, Li B, Yang T, Blackwell DJ, Harvey ML, Smith JE, Chew AE, Capra JA, Matreyek KA, Fowler DM, Roden DM, Glazer AM. bioRxiv. 2023 Apr 29:2023.04.28.538612. doi: 10.1101/2023.04.28.538612. Preprint. PubMed
DNA-guided transcription factor cooperativity shapes face and limb mesenchyme. Kim S, Morgunova E, Naqvi S, Bader M, Koska M, Popov A, Luong C, Pogson A, Claes P, Taipale J, Wysocka J. bioRxiv. 2023 May 29:2023.05.29.541540. doi: 10.1101/2023.05.29.541540. Preprint. PubMed
Discovering chromatin dysregulation induced by protein-coding perturbations at scale. Frenkel M, Hujoel MLA, Morris Z, Raman S. bioRxiv [Preprint]. 2023 Sep 21:2023.09.20.555752. doi: 10.1101/2023.09.20.555752. PubMed
Multi-site validation of a functional assay to adjudicate SCN5A Brugada Syndrome-associated variants. Ma JG, O'Neill MJ, Richardson E, Thomson KL, Ingles J, Muhammad A, Solus JF, Davogustto G, Anderson KC, Benjamin Shoemaker M, Stergachis AB, Floyd BJ, Dunn K, Parikh VN, Chubb H, Perrin MJ, Roden DM, Vandenberg JI, Ng CA, Glazer AM. medRxiv [Preprint]. 2023 Dec 20:2023.12.19.23299592. doi: 10.1101/2023.12.19.23299592. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.