pLenti.tie1.H2B.eYFP.W
(Plasmid #51293)

• Purpose: Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter
• Depositing Lab: Rusty Lansford
• Publication: Sato et al PLoS One. 2010 Sep 14;5(9):e12674. doi: 10.1371/journal.pone.0012674.

Backbone

• Vector backbone: pRRLsin.cPPT.Tie1p.eGFP.wpre
• Backbone manufacturer: Luigi Naldini
• Backbone size w/o insert (bp): 6697
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-eYFP
• Alt name: Histone 2B-enhanced yellow fluorescent protein
• Species: H. sapiens (human)
• Insert Size (bp): 1116
• Entrez Gene: H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
• Promoter: mTie1
• Tag / Fusion Protein:
  • H2B-eYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: BsrGI (destroyed during cloning)
• 5′ sequencing primer: not known
• 3′ sequencing primer: not known

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Original plasmid was a kind gift from Dr. Luigi Naldini

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.