|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51300||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepET SUMO
- Backbone size (bp) 5643
Modifications to backbone1) MultiCloning Site (MCS) of pET28b vector inserted in the pET SUMO cloning site 2) Addition of 2 codon for His residues to the 6 already present in the original vector 3) Removal of the original EcoRI site located inside SUMO ORF 4) Removal of the original HindIII site
Vector typeBacterial Expression
- Promoter T7
/ Fusion Protein
- His8 + SUMO (N terminal on backbone)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Terms and Licenses
This vector is a modified version of the commercial (sell as linearized) pET SUMO vector of Invitrogen. This vector has been optimized for restriction enzyme cloning, thanks to the inserted MultiCloning Site of pET28b.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIOX was a gift from Andrea Mattevi (Addgene plasmid # 51300 ; http://n2t.net/addgene:51300 ; RRID:Addgene_51300)