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Addgene

p35ST
(Plasmid #51514)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51514 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMDC32
  • Backbone size w/o insert (bp) 11752
  • Total vector size (bp) 19573
  • Vector type
    plant expression T-DNA
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    T30 TALEN pair
  • Species
    Xanthomonas
  • Insert Size (bp)
    6600
  • Promoter 2x35S
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • flag (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ccgaattcgcccttcaccatgg
  • 3′ sequencing primer attcgccctttccggaagctg
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    us:NPTII repair template
  • Insert Size (bp)
    2600

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer agcagtcttacttccatgat
  • 3′ sequencing primer tcagaagaactcgtcaagaagg
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    T30 TALEN pair was cloned from pZHY528 into pNJB91. us:nptII repair template was amplified by PCR using pDW1269 and cloned into pNJB80. pNJB80 and pNJB91 were used in a multi-site Gateway reaction with pMDC32.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there may be some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These discrepancies are not in functionally relevant regions of the plasmid. Also, note that Addgene was unable to sequence the RVD region of this plasmid due the repetitive nature of the sequence around it.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p35ST was a gift from Daniel Voytas (Addgene plasmid # 51514 ; http://n2t.net/addgene:51514 ; RRID:Addgene_51514)
  • For your References section:

    DNA Replicons for Plant Genome Engineering. Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF. Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792 10.1105/tpc.113.119792 PubMed 24443519