pRSCgfp-hAIM2
(Plasmid #51666)

• Purpose: Express GFP and hAIM2 in mammalian cells
• Depositing Lab: Emad Alnemri
• Publication: Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21.

Backbone

• Vector backbone: pRSC
• Backbone manufacturer: Unknown
• Backbone size w/o insert (bp): 6470
• Total vector size (bp): 8485
• Modifications to backbone: The vector backbone was created by T.C. Tsang et al (Tsang TC, Harris DT, Akporiaye ET, Chu RS, Brailey J, Liu F, Vasanwala FH, Schluter SF, Hersh EM. Mammalian Expression Vector with Two Multiple Cloning Sites for Expression of Two Foreign Genes. Bio Techniques 22, 68, 1997.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: AIM2
• Species: H. sapiens (human)
• Insert Size (bp): 1032
• Entrez Gene: AIM2 (a.k.a. PYHIN4)
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Gene/Insert 2

• Gene/Insert name: GFP
• Alt name: EGFP
• Species: unknown
• Insert Size (bp): 720
• GenBank ID: unknown
• Promoter: RSV

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry