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pCDNA3-ySet2
(Plasmid #51698)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 +
  • Backbone manufacturer
    commercial
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7600
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeast Set2
  • Alt name
    SETD2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2200
  • GenBank ID
    NC_001142.9 NM_001181601
  • Entrez Gene
    SET2 (a.k.a. YJL168C, EZL1, KMT3)
  • Promoter CMV
  • Tag / Fusion Protein
    • C-terminal FLAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-ySet2 was a gift from Tom Misteli (Addgene plasmid # 51698 ; http://n2t.net/addgene:51698 ; RRID:Addgene_51698)
  • For your References section:

    Regulation of alternative splicing by histone modifications. Luco RF, Pan Q, Tominaga K, Blencowe BJ, Pereira-Smith OM, Misteli T. Science. 2010 Feb 19;327(5968):996-1000. doi: 10.1126/science.1184208. Epub 2010 Feb 4. 10.1126/science.1184208 PubMed 20133523