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pRS307
(Plasmid #51785)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51785 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS306 and pRS317
  • Modifications to backbone
    pRS317 was cleaved at the ApaLI site common to the pRS vectors, the ends were blunted with the Klenow fragment of DNA polymerase, the DNA was cleaved with EcoRI, and the 5.57-kb ApaLI (blunt)/EcoRI fragment was purified. pRS306 was cleaved with NdeI, the ends were blunted with the Klenow fragment of DNA polymerase, cleaved with EcoRI, and the 2.65-kb NdeI (blunt)/EcoRI fragment was purified. The two fragments were ligated to create pRS307.
  • Vector type
    Yeast Expression
  • Selectable markers
    LYS2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS307 was a gift from David Stillman (Addgene plasmid # 51785 ; http://n2t.net/addgene:51785 ; RRID:Addgene_51785)
  • For your References section:

    pRS yeast vectors with a LYS2 marker. Eriksson P, Thomas LR, Thorburn A, Stillman DJ. Biotechniques. 2004 Feb;36(2):212-3. 10.2144/04362BM01 PubMed 14989082