pCS2+hSpCas9 Citations (29)

Originally described in: Targeted mutagenesis using CRISPR/Cas system in medaka.
Ansai S, Kinoshita M Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177. PubMed Journal

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Articles Citing pCS2+hSpCas9

Articles
ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects. Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara-Fujisawa A. Dev Growth Differ. 2016 May;58(4):409-21. doi: 10.1111/dgd.12286. Epub 2016 May 16. PubMed
Targeted candidate gene screens using CRISPR/Cas9 technology. Shah AN, Moens CB, Miller AC. Methods Cell Biol. 2016;135:89-106. doi: 10.1016/bs.mcb.2016.01.008. Epub 2016 Feb 26. PubMed
Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System. Ota S, Kawahara A. Methods Mol Biol. 2016;1451:53-63. doi: 10.1007/978-1-4939-3771-4_4. PubMed
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish. Kawahara A. Methods Mol Biol. 2017;1630:165-173. doi: 10.1007/978-1-4939-7128-2_14. PubMed
Genome Editing of Medaka. Ansai S, Kinoshita M. Methods Mol Biol. 2017;1630:175-188. doi: 10.1007/978-1-4939-7128-2_15. PubMed
Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant. Horimizu R, Ogawa R, Watanabe Y, Tatsukawa H, Kinoshita M, Hashimoto H, Hitomi K. FEBS J. 2017 Jun 27. doi: 10.1111/febs.14153. PubMed
An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Murakami Y, Ansai S, Yonemura A, Kinoshita M. Zoological Lett. 2017 Jul 6;3:10. doi: 10.1186/s40851-017-0071-x. eCollection 2017. PubMed
Using CRISPR/Cas9-mediated gene editing to further explore growth and trade-off effects in myostatin-mutated F4 medaka (Oryzias latipes). Yeh YC, Kinoshita M, Ng TH, Chang YH, Maekawa S, Chiang YA, Aoki T, Wang HC. Sci Rep. 2017 Sep 12;7(1):11435. doi: 10.1038/s41598-017-09966-9. PubMed
Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Watanabe Y, Furukawa E, Tatsukawa H, Hashimoto H, Kamei Y, Taniguchi Y, Hitomi K. Biosci Biotechnol Biochem. 2018 Jul;82(7):1165-1168. doi: 10.1080/09168451.2018.1453294. Epub 2018 Mar 23. PubMed
Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H. PLoS Genet. 2018 Apr 5;14(4):e1007260. doi: 10.1371/journal.pgen.1007260. eCollection 2018 Apr. PubMed
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus. Asakawa K, Kawakami K. Cell Rep. 2018 Aug 7;24(6):1562-1572. doi: 10.1016/j.celrep.2018.07.024. PubMed
Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. Kato-Unoki Y, Takai Y, Kinoshita M, Mochizuki T, Tatsuno R, Shimasaki Y, Oshima Y. Toxicon. 2018 Oct;153:58-61. doi: 10.1016/j.toxicon.2018.08.001. Epub 2018 Aug 29. PubMed
A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish. Shiraki T, Kawakami K. Sci Rep. 2018 Sep 6;8(1):13366. doi: 10.1038/s41598-018-31476-5. PubMed
Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus. Shibata Y, Bao L, Fu L, Shi B, Shi YB. Methods Mol Biol. 2019;1874:507-524. doi: 10.1007/978-1-4939-8831-0_29. PubMed
Nuclear Progestin Receptor Phosphorylation by Cdk9 Is Required for the Expression of Mmp15, a Protease Indispensable for Ovulation in Medaka. Ogiwara K, Takahashi T. Cells. 2019 Mar 4;8(3). pii: cells8030215. doi: 10.3390/cells8030215. PubMed
Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons. Asakawa K, Handa H, Kawakami K. Nat Commun. 2020 Feb 21;11(1):1004. doi: 10.1038/s41467-020-14815-x. PubMed
Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway. Lee YH, Kawakami K, HuangFu WC, Liu IH. PLoS One. 2020 Apr 2;15(4):e0230943. doi: 10.1371/journal.pone.0230943. eCollection 2020. PubMed
Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation. Watanabe Y, Okuya K, Takada Y, Kinoshita M, Yokoi S, Chisada S, Kamei Y, Tatsukawa H, Yamamoto N, Abe H, Hashimoto H, Hitomi K. J Biochem. 2020 Apr 6. pii: 5816600. doi: 10.1093/jb/mvaa038. PubMed
foxl3, a sexual switch in germ cells, initiates two independent molecular pathways for commitment to oogenesis in medaka. Kikuchi M, Nishimura T, Ishishita S, Matsuda Y, Tanaka M. Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):12174-12181. doi: 10.1073/pnas.1918556117. Epub 2020 May 14. PubMed
Male-predominant galanin mediates androgen-dependent aggressive chases in medaka. Yamashita J, Takeuchi A, Hosono K, Fleming T, Nagahama Y, Okubo K. Elife. 2020 Aug 12;9. pii: 59470. doi: 10.7554/eLife.59470. PubMed
Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes. Kato-Unoki Y, Takai Y, Nagano Y, Matsunaga S, Enoki S, Takamura T, Kim S, Kinoshita M, Kitano T, Shimasaki Y, Oshima Y. Mar Pollut Bull. 2020 Nov;160:111601. doi: 10.1016/j.marpolbul.2020.111601. Epub 2020 Aug 30. PubMed
Impact of Gba2 on neuronopathic Gaucher's disease and alpha-synuclein accumulation in medaka (Oryzias latipes). Nakanishi E, Uemura N, Akiyama H, Kinoshita M, Masanori S, Taruno Y, Yamakado H, Matsuzawa SI, Takeda S, Hirabayashi Y, Takahashi R. Mol Brain. 2021 May 10;14(1):80. doi: 10.1186/s13041-021-00790-x. PubMed
Foxq2 determines blue cone identity in zebrafish. Ogawa Y, Shiraki T, Fukada Y, Kojima D. Sci Adv. 2021 Oct 8;7(41):eabi9784. doi: 10.1126/sciadv.abi9784. Epub 2021 Oct 6. PubMed
Sulfation of sialic acid is ubiquitous and essential for vertebrate development. Ertunc N, Phitak T, Wu D, Fujita H, Hane M, Sato C, Kitajima K. Sci Rep. 2022 Jul 21;12(1):12496. doi: 10.1038/s41598-022-15143-4. PubMed
Methylome data derived from maternal-zygotic DNA methyltransferase 3aa(-/-) zebrafish. Shirai M, Takayama K, Takahashi H, Hirose Y, Fujii M, Awazu A, Shimoda N, Kikuchi Y. Data Brief. 2022 Aug 5;44:108514. doi: 10.1016/j.dib.2022.108514. eCollection 2022 Oct. PubMed
Prostaglandin E(2) receptor Ptger4b regulates female-specific peptidergic neurons and female sexual receptivity in medaka. Fleming T, Kikuchi Y, Nakajo M, Tachizawa M, Inazumi T, Tsuchiya S, Sugimoto Y, Saito D, Suyama M, Ohkawa Y, Baba T, Morohashi KI, Okubo K. Commun Biol. 2022 Nov 10;5(1):1215. doi: 10.1038/s42003-022-04195-x. PubMed
Critical Role of the Cortical Alveolus Protease Alveolin in Chorion Hardening In Vivo at Medaka Fertilization. Fu B, Wu D, Yasumasu S, Hane M, Sato C, Kitajima K. Biomolecules. 2023 Jan 10;13(1):146. doi: 10.3390/biom13010146. PubMed
CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain. Altburger C, Holzhauser J, Driever W. Front Neuroanat. 2023 Aug 2;17:1196868. doi: 10.3389/fnana.2023.1196868. eCollection 2023. PubMed
Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos. Kiyomitsu A, Nishimura T, Hwang SJ, Ansai S, Kanemaki MT, Tanaka M, Kiyomitsu T. Nat Commun. 2024 Feb 1;15(1):981. doi: 10.1038/s41467-024-45251-w. PubMed

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