Syn-ATP
(Plasmid #51819)

• Purpose: Optical reporter of presynaptic ATP
• Depositing Lab: Timothy Ryan
• Publication: Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10.1016/j.cell.2013.12.042.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 8700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chimeric Synaptophysin-mCherry-Luciferase
• Alt name: Syn-ATP
• Alt name: TSM2
• Species: R. norvegicus (rat); Photinus pyralis, Discosoma striata
• Insert Size (bp): 3300
• Mutation: Within WT luciferase gene, changed Threonine 214 to Alanine, changed Alanine 215 to Leucine, changed Isoleucine 232 to Alanine, changed Phenylalanine 295 to Leucine, changed Glutamic Acid 354 to Lysine, changed Isoleucine 423 to Leucine, and changed Aspartic Acid 436 to Glycine.
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI, XhoI (not destroyed)
• 5′ sequencing primer: CACTTCTTCATAGTTGGCCGCTTGAAGTCTTTA
• 3′ sequencing primer: TAAAGACTTCAAGCGGCCAACTATGAAGAAGTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mutant luciferase from B.R. Branchini, U. Conn
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry