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pcdna3 EE Jip1b
(Plasmid #52127)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52127 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JNK interacting protein-1b (JIP-1b)
  • Alt name
    JIP-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2152
  • GenBank ID
    AF054611.1
  • Entrez Gene
    Mapk8ip1 (a.k.a. IB1, JIP-1, Jip1, Prkm8ip, Skip, mjip-2a)
  • Tag / Fusion Protein
    • EE (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are a few mismatches between the depositor's reference sequence and Addgene's quality control sequence. The changes are upstream of the JIP1 ORF and should not affect its function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 EE Jip1b was a gift from Roger Davis (Addgene plasmid # 52127 ; http://n2t.net/addgene:52127 ; RRID:Addgene_52127)
  • For your References section:

    A mammalian scaffold complex that selectively mediates MAP kinase activation. Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ. Science. 1998 Sep 11;281(5383):1671-4. 10.1126/science.281.5383.1671 PubMed 9733513