Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pGEX-TEVsite-human Glomulin
(Plasmid #52292)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52292 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Modifications to backbone
    modified to contain a TEV proteolytic cleavage site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Glomulin
  • Species
    H. sapiens (human)
  • Entrez Gene
    GLMN (a.k.a. FAP, FAP48, FAP68, FKBPAP, GLML, GVM, VMGLOM)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • TEV (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-TEVsite-human Glomulin was a gift from Brenda Schulman (Addgene plasmid # 52292 ; http://n2t.net/addgene:52292 ; RRID:Addgene_52292)
  • For your References section:

    Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface. Duda DM, Olszewski JL, Tron AE, Hammel M, Lambert LJ, Waddell MB, Mittag T, DeCaprio JA, Schulman BA. Mol Cell. 2012 Aug 10;47(3):371-82. doi: 10.1016/j.molcel.2012.05.044. Epub 2012 Jun 28. 10.1016/j.molcel.2012.05.044 PubMed 22748924