His Puc (aa 1-393)
(Plasmid #52295)

• Purpose: Bacterial expression of His tagged Drosophila Puc fragment
• Depositing Lab: Brenda Schulman
• Publication: Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.

Backbone

• Vector backbone: pRSFDuet
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Puc
• Species: D. melanogaster (fly)
• Entrez Gene: puc (a.k.a. Dmel_CG7850, 0238/03, 1351/08, CG7850, Dmel\CG7850, JNK-DSP, PUC, PUC/MKP, Puc, hrt, l(3)84Eh, l(3)A251.1, l(3)j4E1, vco)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: LacI-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When compared to GenBank reference sequence NP_524273.1, the Puc insert in this plasmid contains an additional 3 amino acids between aa 309 & 310. The numbering of Puc ending at aa 393 does not count the starting Met but does include the additional 3 amino acids.