pEL333
(Plasmid
#52349)
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Purposeswan-1 co-IP construct
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV-3xFLAG
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6299
- Total vector size (bp) 7472
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameswan-1
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SpeciesC. elegans (nematode)
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Insert Size (bp)1167
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Entrez Geneswan-1 (a.k.a. CELE_F53C11.8)
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEL333 was a gift from Erik Lundquist (Addgene plasmid # 52349 ; http://n2t.net/addgene:52349 ; RRID:Addgene_52349) -
For your References section:
SWAN-1, a Caenorhabditis elegans WD repeat protein of the AN11 family, is a negative regulator of Rac GTPase function. Yang Y, Lu J, Rovnak J, Quackenbush SL, Lundquist EA. Genetics. 2006 Dec;174(4):1917-32. Epub 2006 Sep 15. 10.1534/genetics.106.063115 PubMed 16980389
Map uploaded by the depositor.
