pCLC3sHA
(Plasmid #52424)

• Purpose: mammalian expression of HA tagged rat CLC3 short transcript
• Depositing Lab: Steven Weinman
• Publication: Wang et al Gastroenterology. 2004 Apr;126(4):1157-66.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 6513
• Modifications to backbone: GFP was replaced wtih HA
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CLC3
• Species: R. norvegicus (rat)
• GenBank ID: D17521
• Entrez Gene: Clcn3 (a.k.a. ClC-3)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert represents a portion of the 5’UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.