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pHL 100
(Plasmid #52989)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52989 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    unknown
  • Backbone manufacturer
    Lim lab
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OxyS sRNA
  • Species
    E.coli
  • Promoter pLlac0-1
  • Tag / Fusion Protein
    • RBS-mCherry (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    fhlA
  • Alt name
    target mRNA
  • Species
    E. coli
  • Mutation
    −309 to +96 bp relative to start codon
  • Promoter pLtetO-1
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The transcription of each sRNA was controlled by the pLlacO-1 promoter, which was induced by adding isopropyl-β-d-thiogalactopyranoside (IPTG) to the media. The part of the target mRNA that is necessary for sRNA regulation was fused to gfp and constitutively transcribed from the pLtetO-1 promoter without TetR in the system. GFP fluorescence provides a quantitative measure of target gene expression and sRNA activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL 100 was a gift from Han Lim (Addgene plasmid # 52989 ; http://n2t.net/addgene:52989 ; RRID:Addgene_52989)
  • For your References section:

    Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298