JF001A
(Plasmid #53116)

• Purpose: expresses alpha hemolysin under the control of theophylline riboswitch
• Depositing Lab: Sheref Mansy
• Publication: Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.1038/ncomms5012.

Backbone

• Vector backbone: pET21b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5442
• Total vector size (bp): 6278
• Modifications to backbone: lacO and original RBS were removed from pET21b and substituted with the theophylline riboswitch
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: theophylline riboswitch and alpha hemolysin
• Alt name: alpha hemolysin from Staphylococcus aureus
• Alt name: αHL
• Species: Staphylococcus aureus
• Insert Size (bp): 939
• Promoter: T7 promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GGCAGCACCCTGCTAAGGTAACAACAAG ATGGATTCTGATATCAATATCAAAACC
• 3′ sequencing primer: AAG GGC ATC AAG ACG ATG CTG GTA TCA CCC TAT AGT GAG TCG TAT TAA TTT CGC GG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry