SNV-gfp-IRES-MCS
(Plasmid #53122)

• Purpose: (Empty Backbone) Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
• Depositing Lab: Jeanette Hyer
• Publication: Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766.

Backbone

• Vector backbone: pBMN-LACZ
• Backbone manufacturer: Nolan Lab
• Backbone size (bp): 5810
• Vector type: Retroviral ; for expression in chicken cells
• Promoter: SNV (spleen necrosis virus)
• Tag / Fusion Protein:
  • EGFP-IRES (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: IRES-F
• 3′ sequencing primer: LNCX-3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MCS is ClaI and SalI.
This is a pro-retroviral vector for expression in chick tissue. It is based on the Pheonix system of Gary Nolan, Stanford University. The virus is packaged using Pheonix cells and VSVg pseudotyped. However, because of inclusion of a second, chicken promoter, it does not express well in mammalian cells. Can be titered using Dog osteosarcoma (D17) cells. Replication incompetent in chicken. Gives exceptionally bright GFP signal, suitable for live imaging and lineage tracing.
This plasmid is unstable during transformation. If doing a fresh transformation, confirm correct size by digest (correct size is 6kb) before proceeding further.