mCit-4aa-SNAP25B
(Plasmid #53235)

• Purpose: Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.
• Depositing Lab: Robert Zucker
• Publication: Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013.

Backbone

• Vector backbone: pECFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5333
• Modifications to backbone: ECFP has been substituted by citrine, which was further made monomeric by introducing the A206K mutation using the Stratagene QuikChange Site-Directed Mutagenesis Kit, and exchanged with EYFP by digestion with restriction endonucleases after ligation.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SNAP-25B
• Alt name: synaptosomal-associated protein, 25kDa
• Species: R. norvegicus (rat)
• Insert Size (bp): 621
• GenBank ID: AB003992 AB003992.1
• Entrez Gene: Snap25 (a.k.a. SNAP-25B, SNAP-25a)
• Tags / Fusion Proteins:
  • mCitrine (N terminal on backbone)
  • 4aa-linker (N terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry