Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

mCer-Vamp2(qv/fw)
(Plasmid #53238)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53238 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5063
  • Modifications to backbone
    This vector was created by mutagenesis of pECFP-C1. Compared to ECFP, Cerulean contains mutations: S72A, Y145A and H148D. mCerulean also contains A206K mutation to create a monomeric form of the fluorescent protein. See more details in Addgene's plasmid 27796.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP2
  • Alt name
    Vesicle-Associated Membrane Protein 2
  • Alt name
    synaptobrevin 2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    351
  • Mutation
    Mutated Q76 to V; F77 to W.
  • GenBank ID
    NM_012663
  • Entrez Gene
    Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
  • Tags / Fusion Proteins
    • mCerulean (N terminal on backbone)
    • 4aa-linker (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCer-Vamp2(qv/fw) was a gift from Robert Zucker (Addgene plasmid # 53238 ; http://n2t.net/addgene:53238 ; RRID:Addgene_53238)
  • For your References section:

    Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. Degtyar V, Hafez IM, Bray C, Zucker RS. J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. 10.1523/JNEUROSCI.2337-12.2013 PubMed 23536066