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pET28a/Cas9-Cys Citations (18)

Originally described in: Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.
Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H Genome Res. 2014 Apr 2.
PubMed Journal

Articles Citing pET28a/Cas9-Cys

Articles
Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture. Lee N, Shin J, Park JH, Lee GM, Cho S, Cho BK. ACS Synth Biol. 2016 Nov 18;5(11):1211-1219. Epub 2016 Feb 18. PubMed
CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum. Pohl C, Kiel JA, Driessen AJ, Bovenberg RA, Nygard Y. ACS Synth Biol. 2016 Jul 15;5(7):754-64. doi: 10.1021/acssynbio.6b00082. Epub 2016 May 3. PubMed
An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein. Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M, Blin C, Finch R, Bressan RB, Morrison G, Jacobi AM, Behlke MA, von Kriegsheim A, Tomlinson S, Krijgsveld J, Pollard SM. Elife. 2018 Apr 11;7. pii: 35069. doi: 10.7554/eLife.35069. PubMed
Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering. Shin J, Lee N, Cho S, Cho BK. Methods Mol Biol. 2018;1772:151-169. doi: 10.1007/978-1-4939-7795-6_8. PubMed
Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing. Chaverra-Rodriguez D, Macias VM, Hughes GL, Pujhari S, Suzuki Y, Peterson DR, Kim D, McKeand S, Rasgon JL. Nat Commun. 2018 Aug 1;9(1):3008. doi: 10.1038/s41467-018-05425-9. PubMed
A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection. Zhou W, Hu L, Ying L, Zhao Z, Chu PK, Yu XF. Nat Commun. 2018 Nov 27;9(1):5012. doi: 10.1038/s41467-018-07324-5. PubMed
CRISPR/Cas9-generated mouse model of Duchenne muscular dystrophy recapitulating a newly identified large 430 kb deletion in the human DMD gene. Egorova TV, Zotova ED, Reshetov DA, Polikarpova AV, Vassilieva SG, Vlodavets DV, Gavrilov AA, Ulianov SV, Buchman VL, Deykin AV. Dis Model Mech. 2019 Apr 25;12(4). pii: 12/4/dmm037655. doi: 10.1242/dmm.037655. PubMed
Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome. Forsberg KJ, Bhatt IV, Schmidtke DT, Javanmardi K, Dillard KE, Stoddard BL, Finkelstein IJ, Kaiser BK, Malik HS. Elife. 2019 Sep 10;8. pii: 46540. doi: 10.7554/eLife.46540. PubMed
Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi. Kwon MJ, Schutze T, Spohner S, Haefner S, Meyer V. Fungal Biol Biotechnol. 2019 Oct 17;6:15. doi: 10.1186/s40694-019-0079-4. eCollection 2019. PubMed
Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice. Ali Z, Shami A, Sedeek K, Kamel R, Alhabsi A, Tehseen M, Hassan N, Butt H, Kababji A, Hamdan SM, Mahfouz MM. Commun Biol. 2020 Jan 23;3(1):44. doi: 10.1038/s42003-020-0768-9. PubMed

Associated Plasmids

Purification of Cas9-RNA complexes by ultrafiltration. Manzano I, Taylor N, Csordas M, Vezeau GE, Salis HM, Zydney AL. Biotechnol Prog. 2021 Mar;37(2):e3104. doi: 10.1002/btpr.3104. Epub 2020 Dec 14. PubMed
CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM. Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. PubMed

Associated Plasmids

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion. Gangoso E, Southgate B, Bradley L, Rus S, Galvez-Cancino F, McGivern N, Guc E, Kapourani CA, Byron A, Ferguson KM, Alfazema N, Morrison G, Grant V, Blin C, Sou I, Marques-Torrejon MA, Conde L, Parrinello S, Herrero J, Beck S, Brandner S, Brennan PM, Bertone P, Pollard JW, Quezada SA, Sproul D, Frame MC, Serrels A, Pollard SM. Cell. 2021 Apr 29;184(9):2454-2470.e26. doi: 10.1016/j.cell.2021.03.023. Epub 2021 Apr 14. PubMed
Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma. Cui H, Jiang M, Zhou W, Gao M, He R, Huang Y, Chu PK, Yu XF. Biology (Basel). 2021 Oct 13;10(10). pii: biology10101038. doi: 10.3390/biology10101038. PubMed
CRISPA: A Non-viral, Transient Cas9 Delivery System Based on Reengineered Anthrax Toxin. Hirschenberger M, Stadler N, Fellermann M, Sparrer KMJ, Kirchhoff F, Barth H, Papatheodorou P. Front Pharmacol. 2021 Oct 18;12:770283. doi: 10.3389/fphar.2021.770283. eCollection 2021. PubMed
One-shot dual gene editing for drug-resistant pancreatic cancer therapy. Won EJ, Park H, Chang SH, Kim JH, Kwon H, Cho YS, Yoon TJ. Biomaterials. 2021 Dec;279:121252. doi: 10.1016/j.biomaterials.2021.121252. Epub 2021 Nov 12. PubMed
CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the Gnao1 for safety studies of RNA therapeutics. Polikarpova AV, Egorova TV, Lunev EA, Tsitrina AA, Vassilieva SG, Savchenko IM, Silaeva YY, Deykin AV, Bardina MV. Front Genome Ed. 2023 Apr 3;5:1034720. doi: 10.3389/fgeed.2023.1034720. eCollection 2023. PubMed
In-Frame Deletion of Dystrophin Exons 8-50 Results in DMD Phenotype. Egorova TV, Galkin II, Velyaev OA, Vassilieva SG, Savchenko IM, Loginov VA, Dzhenkova MA, Korshunova DS, Kozlova OS, Ivankov DN, Polikarpova AV. Int J Mol Sci. 2023 May 23;24(11):9117. doi: 10.3390/ijms24119117. PubMed

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