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pAC-LYCipi
(Plasmid #53279)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53279 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAC-LYC
  • Backbone manufacturer
    Francis X. Cunningham, Jr.
  • Backbone size w/o insert (bp) 9476
  • Total vector size (bp) 8916
  • Modifications to backbone
    Extraneous sequence was removed from pAC-LYC by digestion with SphI and DrdI, blunting with mung bean nuclease, and recircularization with ligation. This 7.8-kb plasmid was then digested with HindIII and EcoRV to yield a 6.4-kB fragment that was ligated with a HindIII + mung bean-blunted DraIII fragment of 2.55 kB obtained from pAC-EHER.
  • Vector type
    low copy number bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness for best lycopene production, or grow on agar plates at room temperature for 3-7 days.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    idi
  • Alt name
    ORF6
  • Species
    Erwinia herbicola Eho10
  • Insert Size (bp)
    2750
  • GenBank ID
    M87280.1
  • Promoter endogenous promoters

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-LYCipi was a gift from Francis X Cunningham Jr (Addgene plasmid # 53279 ; http://n2t.net/addgene:53279 ; RRID:Addgene_53279)
  • For your References section:

    Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon merolae. Cunningham FX Jr, Lee H, Gantt E. Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3. 10.1128/EC.00265-06 PubMed 17085635