EGFP-mOrange pHis-//
(Plasmid
#53302)
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PurposeExpresses His-tagged EGFP-mOrange fusion protein.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHis-parallel
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEGFP
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- mOrange (C terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-mOrange pHis-// was a gift from John Lis (Addgene plasmid # 53302 ; http://n2t.net/addgene:53302 ; RRID:Addgene_53302) -
For your References section:
Comprehensive analysis of RNA-protein interactions by high-throughput sequencing-RNA affinity profiling. Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT. Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 2014 May 8. 10.1038/nmeth.2970 PubMed 24809628
Map uploaded by the depositor.
