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pDY4
(Plasmid #53589)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53589 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2958
  • Total vector size (bp) 4762
  • Modifications to backbone
    This is a cDNA library plasmid containing a Lactuca sativa (romaine lettuce) lycopene epsilon-cyclase cDNA. This plasmid has been referred to elsewhere as pLsLCYe [Cunningham FX Jr, Gantt E (2001) A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Photosynth Res. 92, 245-259.].
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Can be used with pAC-LYC or pAC-LYCipi in E. coli strain TOP10 to produce epsilon-carotene. For this purpose, grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness, or grow on agar plates at room temperature for 3-7 days.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lcyE
  • Alt name
    DY4
  • Alt name
    lycopene epsilon-cyclase
  • Alt name
    lycopene epsilon-bicyclase
  • Species
    Lactuca sativa
  • Insert Size (bp)
    1780
  • GenBank ID
    AF321538.1
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was selected in a screen of a Lactuca sativa (Romaine lettuce) cDNA library. The library was kindly provided to us by Harry Yamamoto and Bob Bugos of the University of Hawaii [see Bugos RC, Yamamoto HY (1996) Molecular cloning of violaxanthin de-epoxidase from romaine lettuce and expression in Escherichia coli. Proc Natl Acad Sci USA. 93, 6320-6325.].
  • Terms and Licenses

Depositor Comments

Please note that the DY4 insert in this plasmid contains an extra aspartic acid, D178, compared to GenBank reference sequence AF321538.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDY4 was a gift from Francis X Cunningham Jr (Addgene plasmid # 53589 ; http://n2t.net/addgene:53589 ; RRID:Addgene_53589)
  • For your References section:

    One ring or two? Determination of ring number in carotenoids by lycopene epsilon-cyclases. Cunningham FX Jr, Gantt E. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2905-10. Epub 2001 Feb 20. 10.1073/pnas.051618398 PubMed 11226339