pMGIB-3xFLAG-pontin
(Plasmid #53608)

• Purpose: Retroviral expression of human pontin
• Depositing Lab: Steven Artandi
• Publication: Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.

Backbone

• Vector backbone: pMGIB
• Backbone manufacturer: -
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7000
• Modifications to backbone: Retroviral backbone. Insert followed by IRES-Blasticidin cassette
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pontin
• Alt name: RUVBL1
• Alt name: RuvB-like AAA ATPase 1
• Species: H. sapiens (human)
• Mutation: pontin CDS mutated to confer resistance to shRNA. All mutations silent
• GenBank ID: NM_003707.2; NP_003698.1
• Entrez Gene: RUVBL1 (a.k.a. ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A)
• Promoter: LTR
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: IRES-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry