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pcDNA4-ATR-C-KD
(Plasmid #53770)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53770 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATR
  • Alt name
    ataxia telangiectasia and Rad3 related
  • Species
    H. sapiens (human)
  • Mutation
    C-terminal domain, aa 1398-2644, and kinase dead mutation (K2327R)*
  • Entrez Gene
    ATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

*R1631H polymorphism--not known to affect function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-ATR-C-KD was a gift from Aziz Sancar (Addgene plasmid # 53770 ; http://n2t.net/addgene:53770 ; RRID:Addgene_53770)
  • For your References section:

    Preferential binding of ATR protein to UV-damaged DNA. Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8. 10.1073/pnas.102167799 PubMed 12011431