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pKSII/INSeGFP
(Plasmid #53787)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53787 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 4937
  • Modifications to backbone
    Zebrafish insulin promoter DNA was derived from genomic sequence, eGFP and SV40pA were inserted at engineered Nco1 site. Flanking EcoRV sites remove cassette for insertion into pTC3 expression vector (Curr Biol. 1998 Jan 15;8(2):82-8)
  • Vector type
    Bacterial Expression ; zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INS promoter/eGFP/pA
  • Alt name
    ZIP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    979
  • Promoter zebrafish insulin/ZIP
  • Tag / Fusion Protein
    • eGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note: Addgene's quality control sequencing finds a few discrepancies with the expected sequence of this plasmid. However, the depositing laboratory believes that this plasmid accurately represents the construct as it was used in the associated publication, and it should function as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKSII/INSeGFP was a gift from Larry Moss (Addgene plasmid # 53787 ; http://n2t.net/addgene:53787 ; RRID:Addgene_53787)
  • For your References section:

    Sonic hedgehog is required early in pancreatic islet development. diIorio PJ, Moss JB, Sbrogna JL, Karlstrom RO, Moss LG. Dev Biol. 2002 Apr 1;244(1):75-84. 10.1006/dbio.2002.0573 PubMed 11900460