Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)
(Plasmid #53959)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53959 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript2 SK+
  • Modifications to backbone
    see author map
  • Vector type
    Mammalian Expression, Cre/Lox
  • Promoter CMV enhancer, chicken actin promoter
  • Tag / Fusion Protein
    • Lox-CAT-Lox (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When the CAT gene is excised by Cre-mediated recombination between the tandem loxP sites, the gene of interest becomes positioned adjacent to the CAG promoter, permitting gene expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17) was a gift from Jeffrey Robbins (Addgene plasmid # 53959 ; http://n2t.net/addgene:53959 ; RRID:Addgene_53959)
  • For your References section:

    Noonan syndrome is associated with enhanced pERK activity, the repression of which can prevent craniofacial malformations. Nakamura T, Gulick J, Pratt R, Robbins J. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15436-41. doi: 10.1073/pnas.0903302106. Epub 2009 Aug 24. 10.1073/pnas.0903302106 PubMed 19706403