PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54304||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 487; Emission = 509. Localization data: Vinculin - Focal Adhesions
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-Vinculin-N-21 was a gift from Michael Davidson (Addgene plasmid # 54304 ; http://n2t.net/addgene:54304 ; RRID:Addgene_54304)
For your References section:A role for actin arcs in the leading-edge advance of migrating cells. Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, Kachar B, Lippincott-Schwartz J. Nat Cell Biol. 2011 Apr;13(4):371-81. doi: 10.1038/ncb2205. Epub 2011 Mar 20. 10.1038/ncb2205 PubMed 21423177