pBS SK mCherryROSAegfp
PurposeEncodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54322||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBS SK(+)
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 5139
Vector typeMammalian Expression, Mouse Targeting, CRISPR, TALEN
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemCherry and EGFP
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS SK mCherryROSAegfp was a gift from Silvestro Conticello (Addgene plasmid # 54322 ; http://n2t.net/addgene:54322 ; RRID:Addgene_54322)
For your References section:An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. 10.1007/s00018-017-2524-y [pii] PubMed 28421278