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mCitrine-N1
(Plasmid #54594)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54594 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mCitrine-N1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • mCitrine (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mCitrine (C terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    R.E. Campbell, R.Y. Tsien, O. Griesbeck; PMID 11387331
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

Citrine + A206K. Excitation = 516; Emission = 529

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCitrine-N1 was a gift from Robert Campbell & Michael Davidson & Oliver Griesbeck & Roger Tsien (Addgene plasmid # 54594)
  • For your References section:

    Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. M102815200 [pii] PubMed 11387331