mKate1.3-Peroxisomes-2
(Plasmid
#54643)
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Purpose(Empty Backbone) Localization: Peroxisomes, Excitation: 588, Emission: 635
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 54643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonemKate1.3
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- mKate1.3 (C terminal on backbone)
- Peroximal Targeting Signal 1 (PTS1) (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mKate1.3 (C terminal on backbone)
- Peroximal Targeting Signal 1 (PTS1) (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mKate1.3 = mKate2. Excitation = 588; Emission = 635
Addgene sequencing results find D224N and R232K compared to mKate2 reference sequence AEX25288.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKate1.3-Peroxisomes-2 was a gift from Michael Davidson (Addgene plasmid # 54643) -
For your References section:
Far-red fluorescent tags for protein imaging in living tissues. Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949. 10.1042/BJ20081949 PubMed 19143658