This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24th & 25th and December 31st & January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Citrine-pBAD
(Plasmid #54772)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54772 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Citrine-pBAD
  • Backbone size (bp) 4800
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • Citrine (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • Citrine (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Resource Information

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 516; Emission = 529

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Citrine-pBAD was a gift from Robert Campbell & Michael Davidson & Oliver Griesbeck & Roger Tsien (Addgene plasmid # 54772 ; http://n2t.net/addgene:54772 ; RRID:Addgene_54772)
  • For your References section:

    Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. M102815200 [pii] PubMed 11387331