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mApple-MyosinIIA-C-18
(Plasmid #54929)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54929 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mApple-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyosinIIA
  • Alt name
    AJ312390
  • Species
    M. musculus (mouse)
  • Promoter CMV
  • Tag / Fusion Protein
    • mApple (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

. Excitation = 568; Emission = 592

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mApple-MyosinIIA-C-18 was a gift from Michael Davidson (Addgene plasmid # 54929)
  • For your References section:

    A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, Lippincott-Schwartz J. J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. Epub 2014 Apr 7. 10.1083/jcb.201311104 PubMed 24711500