PurposeLocalization: Membrane/Actin, Excitation: 587, Emission: 610
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55136||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesB. taurus (bovine)
- Promoter CMV
/ Fusion Protein
- mCherry (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 587; Emission = 610
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCherry-Supervillin-45 was a gift from Michael Davidson (Addgene plasmid # 55136)
For your References section:The membrane-associated protein, supervillin, accelerates F-actin-dependent rapid integrin recycling and cell motility. Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, Lambright DG, Luna EJ. Traffic. 2010 Jun;11(6):782-99. doi: 10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17. 10.1111/j.1600-0854.2010.01062.x PubMed 20331534