Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

mRFP-mem
(Plasmid #55622)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55622 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Derived from pEYFP-Mem
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3942
  • Total vector size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP-Mem
  • Species
    Discosoma sp.
  • Insert Size (bp)
    808
  • Promoter CMV
  • Tag / Fusion Protein
    • The N-terminal 20 residues of neuromodulin are fused to the N-terminus of mRFP.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer sV40pA-Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mRFP1/pcDNA3 was obtained from Roger Tsien, University of California, San Diego. pEYFP-Mem was purchased from Clontech.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for mRFP:
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, et al. A monomeric red fluorescent protein. Proc Natl Acad Sci U S A. 2002;99(12):7877-82. PubMed PMID: 12060735.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-mem was a gift from Catherine Berlot (Addgene plasmid # 55622 ; http://n2t.net/addgene:55622 ; RRID:Addgene_55622)
  • For your References section:

    Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits. Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26. 10.1124/mol.106.022616 PubMed 16641313