pcDNATM3.1/V5-His TOPO-PKCi-K274W
(Plasmid #55690)

• Purpose: expresses dominant negative human PKCi tagged with V5
• Depositing Lab: Pedro Salas
• Publication: Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54. doi: 10.1242/jcs.016246. Epub 2008 Feb 12.

Backbone

• Vector backbone: pcDNA3.1/V5-His TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5523
• Total vector size (bp): 7313
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens protein kinase C, iota (PRKCI)
• Species: H. sapiens (human)
• Insert Size (bp): 1790
• Mutation: A120E and K274W
• GenBank ID: NM_002740.4 NP_002731.3
• Entrez Gene: PRKCI (a.k.a. DXS1179E, PKCI, nPKC-iota)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5/His (C terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

K274W is a kinase dead mutation.