pBacPAK p110a WT
PurposeHis3 GST tagged WT p110 alpha
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55722||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep110 alpha
SpeciesM. musculus (mouse)
Entrez GenePik3ca (a.k.a. 6330412C24Rik, caPI3K, p110, p110alpha)
- Promoter polyhedrin promoter
/ Fusion Protein
- His3 GST (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
murine p110α and p110β constructs (wild type and RBD DM) subcloned into pBacPAK-His3-GST (from protein purification facility). So these constructs encode GST-tagged p110, which can be cleaved of using rhinovirus 3C protease
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBacPAK p110a WT was a gift from Julian Downward (Addgene plasmid # 55722 ; http://n2t.net/addgene:55722 ; RRID:Addgene_55722)
For your References section:RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms. Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, Diefenbacher M, Stamp G, Downward J. Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031. 10.1016/j.cell.2013.04.031 PubMed 23706742