pBacPAK p110a RBD DM
(Plasmid #55723)

• Purpose: His3 GST tagged RBD double mutant p110 alpha T207D, K227A
• Depositing Lab: Julian Downward
• Publication: Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.

Backbone

• Vector backbone: pBacPAK
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5500

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p110 alpha
• Alt name: PI3K
• Species: M. musculus (mouse)
• Mutation: T207D, K227A
• Entrez Gene: Pik3ca (a.k.a. 6330412C24Rik, caPI3K, p110, p110alpha)
• Promoter: polyhedrin promoter
• Tag / Fusion Protein:
  • His3 GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: Polyhedrin forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

murine p110α and p110β constructs (wild type and RBD DM) subcloned into pBacPAK-His3-GST (from protein purification facility). So these constructs encode GST-tagged p110, which can be cleaved of using rhinovirus 3C protease