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pSANG10-3F-BG4
(Plasmid #55756)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55756 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSANG10-3F
  • Backbone manufacturer
    John McCafferty
  • Backbone size w/o insert (bp) 5438
  • Total vector size (bp) 6197
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Must be transformed into BL21(DE3) bacteria and grown at 30 degrees for antibody production.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BG4
  • Alt name
    BG4 anti-G-quadruplex scFv antibody
  • Species
    human phage display antibody from Sanger library
  • Insert Size (bp)
    759
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • tri-FLAG (C terminal on backbone)
    • pelB leader (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Ncol (not destroyed)
  • 3′ cloning site Notl (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminal primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSANG10-3F-BG4 was a gift from Shankar Balasubramanian (Addgene plasmid # 55756 ; http://n2t.net/addgene:55756 ; RRID:Addgene_55756)
  • For your References section:

    Quantitative visualization of DNA G-quadruplex structures in human cells. Biffi G, Tannahill D, McCafferty J, Balasubramanian S. Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1038/nchem.1548. Epub 2013 Jan 20. 10.1038/nchem.1548 PubMed 23422559