Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

CFP(159-238)-beta-5 in pcDNAI/Amp
(Plasmid #55763)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55763 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 6097
  • Modifications to backbone
    BglII introduced to polylinker of pcDNA1/amp 3' of BamHI site.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP(159-238)-beta-5
  • Alt name
    gnb5
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    1297
  • Mutation
    CFP(159-238) includes a substitution of His for Asn-164. GBeta-5 was amplified via PCR, which added an N-terminal linker sequence (RSIAT), a 5' BamHI site, and a 3' BglII site. Cloning into the BglII site of CFP(1-158) destroyed the BamHI site.
  • Entrez Gene
    GNB5 (a.k.a. GB5, HG2E, IDDCA, LADCI, LDMLS1, LDMLS2, gbeta5)
  • Promoter CMV
  • Tag / Fusion Protein
    • CFP(159-238) was fused to the amino terminus of Gbeta5. (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pECFP was purchased from Clontech. Beta-5 cDNA was obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville, PA.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CFP(159-238)-beta-5 in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 55763 ; http://n2t.net/addgene:55763 ; RRID:Addgene_55763)
  • For your References section:

    Live cell analysis of G protein beta5 complex formation, function, and targeting. Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27. 10.1124/mol.107.038075 PubMed 17596375