Dyn1(K44A)-mRFP
(Plasmid #55795)

• Purpose: Dominant negative Dynamin 1 mutant tagged with mRFP
• Depositing Lab: Catherine Berlot
• Publication: Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.

Backbone

• Vector backbone: Derived from pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3950
• Total vector size (bp): 7286
• Modifications to backbone: GFP was removed as a BamHI/NotI cassette and replaced with mRFP.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dynamin-1(K44A)-mRFP
• Alt name: DNM1
• Species: R. norvegicus (rat); Discosoma sp.
• Insert Size (bp): 3336
• Mutation: Dynamin-1(K44A)-mRFP was produced from Dynamin-1(K44A)-GFP by replacing GFP with mRFP.
• GenBank ID:
• Entrez Gene: Dnm1 (a.k.a. Dnm)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dyn1(K44A)-GFP was obtained from Pietro De Camilli, Howard Hughes Medical Institute, Yale University, New Haven, CT. mRFP was obtained from Roger Tsien, University of California, San Diego, California.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry