pLIB-TetR-KRAB-IRES-BLAS
(Plasmid #55820)

• Purpose: retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance
• Depositing Lab: Stephan Geley
• Publication: Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014.

Backbone

• Vector backbone: pLIB
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4694
• Total vector size (bp): 6700
• Modifications to backbone: introduction of a multiple cloning site, an internal ribosomal entry site derived from EMCV and a Blastidicin S resistance gene
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TetR-KRAB
• Species: Synthetic; bacterial/mammalian
• Insert Size (bp): 700
• Promoter: LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n.a.
• 3′ sequencing primer: n.a.

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCMV-TetR-KRAB (Deuschle U., 1995; MCB 15:1907-1914)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry