PurposeLocalization: Actin/Focal Adhesions, Excitation: 586, Emission: 632
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||56049||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationA209T in VASP
- Promoter CMV
/ Fusion Protein
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 586; Emission = 632
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:tdKatushka2-VASP-5 was a gift from Michael Davidson (Addgene plasmid # 56049 ; http://n2t.net/addgene:56049 ; RRID:Addgene_56049)
For your References section:Far-red fluorescent tags for protein imaging in living tissues. Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949. 10.1042/BJ20081949 PubMed 19143658