pRSETC PITPbeta N89F
(Plasmid
#58279)
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Purposebacterial expression of PITP beta N89F mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSET-C
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePITP beta
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Alt namephosphatidylinositol transfer protein
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SpeciesH. sapiens (human)
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MutationN89F (abolishes PI binding)
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Entrez GenePITPNB (a.k.a. PI-TP-beta, PtdInsTP, VIB1B)
- Promoter T7
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Tag
/ Fusion Protein
- HIS (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC sequencing has found some mutations in the insert, which should not effect the function of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETC PITPbeta N89F was a gift from Shamshad Cockcroft (Addgene plasmid # 58279 ; http://n2t.net/addgene:58279 ; RRID:Addgene_58279) -
For your References section:
Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum. Carvou N, Holic R, Li M, Futter C, Skippen A, Cockcroft S. J Cell Sci. 2010 Apr 15;123(Pt 8):1262-73. doi: 10.1242/jcs.061986. Epub 2010 Mar 23. 10.1242/jcs.061986 PubMed 20332109