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pAAV-mCherry-flex-dtA
(Plasmid #58536)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58536 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5604
  • Total vector size (bp) 6780
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dtA
  • Alt name
    diphtheria toxin A
  • Insert Size (bp)
    657
  • GenBank ID
    X00703.1 AB610405.1
  • Entrez Gene
    DIP_RS18250 (a.k.a. DIP_RS18250, DIP1414)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer agcaacatagttaagaatac
  • 3′ sequencing primer cgagcttttggagtacgtcg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-mCherry-flex-dtA was a gift from Naoshige Uchida (Addgene plasmid # 58536 ; http://n2t.net/addgene:58536 ; RRID:Addgene_58536)
  • For your References section:

    Galanin neurons in the medial preoptic area govern parental behaviour. Wu Z, Autry AE, Bergan JF, Watabe-Uchida M, Dulac CG. Nature. 2014 May 15;509(7500):325-30. doi: 10.1038/nature13307. 10.1038/nature13307 PubMed 24828191