pLKO.1 shTGFBR3 puro
(Plasmid #58696)

• Purpose: Lentiviral shRNA vector for knockdown of human TGFBR3
• Depositing Lab: Kevin Janes
• Publication: Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23.

Backbone

• Vector backbone: pLKO.1
• Backbone manufacturer: Weinberg Lab (Addgene plasmid 8453)
• Total vector size (bp): 7032
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shTGFBR3
• gRNA/shRNA sequence: ccggCCAAGCATGAAGGAACCAAATctcgagATTTGGTTCCTTCATGCTTGGtttttg
• Species: H. sapiens (human)
• GenBank ID: NM_001195683
• Entrez Gene: TGFBR3 (a.k.a. BGCAN, betaglycan)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct was obtained from the TRC (#TRCN0000033430) and potently knocks down TGFBR3 expression after 3 x 500 microL infection in MCF10A cells.