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pRS406-PHO5-GFP-CERT PH domain
(Plasmid #58725)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58725 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS406
  • Backbone manufacturer
    Stratagene
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    collagen, type IV, alpha 3 binding protein PH domain
  • Alt name
    CERT
  • Alt name
    GPBP
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001130105.1
  • Entrez Gene
    COL4A3BP (a.k.a. CERT, CERTL, GPBP, MRD34, STARD11)
  • Promoter PHO5
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • Myc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PH domain of CERT= Methionine 1 - Glycine 122

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS406-PHO5-GFP-CERT PH domain was a gift from Tim Levine (Addgene plasmid # 58725 ; http://n2t.net/addgene:58725 ; RRID:Addgene_58725)
  • For your References section:

    Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Levine TP, Munro S. Curr Biol. 2002 Apr 30;12(9):695-704. S0960982202007790 [pii] PubMed 12007412